A second Sydney performance by US comedian Jerry Seinfeld has been interrupted by pro-Palestinian protesters.

Tuesday night's show at ICC Sydney Theatre was paused while the demonstrators holding a Palestinian flag were escorted out of the venue after they heckled the Jewish actor.

Two members of the audience shouted out and waved a Palestinian flag before security intervened while some members of the audience booed.

It is not clear from footage of the incident what the protesters said during the show.

Seinfeld responded to the protesters from the stage, acknowledging their "strong political feelings".

"But you don't know where to say them," he said to the pair.

"You think that ruining the night … it doesn't affect me … all these people, you're ruining their night."

He then made light of the incident before adding: "I love them, too, they just need a little direction of, you know, where to use their tremendous brain power."

On Sunday night, his first Sydney show was also interrupted with videos from social media showing pro-Palestinian chants, including "From the River to the Sea, Palestine will be free" before those members of the audience were escorted out.

Some supporters of Israel consider it as a call for the eradication of the nation but others, including many Palestinians, argue it is simply a call for freedom.

Seinfeld has been outspoken in his support for Israel since the October 7 Hamas attack in which more than 1,100 people were killed, with dozens still help captive, according to Israeli officials.

Two months later, Seinfeld went on a solidarity trip to Israel.

Since October 7, more than 37,300 Palestinians have been killed and almost 85,500 have been injured, according to the Gaza Health Ministry.

In May, dozens of students left their own graduation during his commencement speech at North Carolina's Duke University over his support for Israel, leaving the football field while chanting "free Palestine".