Queensland's Premier Annastacia Palaszczuk says she doesn't mind being an underdog, almost a year out from the next state election.

Ms Palaszczuk says Labor has "climbed Mount Everest" to win power in the past and called on her party to work together.

"The last two elections have shown us that we're unstoppable when we work together," Ms Palaszczuk said at the Queensland ALP conference on Sunday.

The call for unity follows weeks of attacks on her deputy, Jackie Trad, who has faced calls to resign from the state opposition and even the fellow Left-aligned CFMEU over her referral to the state's corruption watchdog last month.