Queensland's treaty minister says an accusation by the LNP of "segregation" in her office is "racist and defamatory". 

Shadow minister for Aboriginal and Torres Strait Islander Partnerships John-Paul Langbroek told estimates that a whistleblower had told the LNP Leeanne Enoch held meetings for First Nations staff only.

Asked if she could confirm this, Ms Enoch said: "Yes, and for the member's cultural awareness training today, can I make it clear that that is part of a cultural practice that happens," Ms Enoch replied.

"Many of the members of my department could be relatives of mine so there are some conversations that need to be had. 

"It is always a standard cultural practice to understand your relationship with Aboriginal and Torres Strait Islander people – whether you have a relationship, as in a familial relationship or other."

Ms Enoch told the hearing it was a way to gauge any potential conflicts of interest and it was something she had done since becoming a minister in the Palaszczuk government.

"It may be a bit unusual for departments to fully appreciate that those cultural practices are being upheld," she said.

Mr Langbroek described it as "segregated meetings".

The department's director-general Clare O'Connor said the minister addressed all staff and if she wished to speak to First Nations employees from time to time, "personally, I don't have a problem with that".

"From time to time, I'll speak to different cohorts of staff as well … I'll speak to women at things like International Women's Day … I might speak to specialist staff."

'Fully transparent' 

Ms Enoch said she had been "fully transparent" with the Integrity Commission about the meetings. 

"I just want to refute some of the suggestions from the member that there are 'segregated meetings happening'," she said.

"I have to undertake cultural practice to understand my relationship in case there is any conflicts of interest so I can address them with the integrity commissioner.

"I've been fully transparent with the integrity commissioner … and been given advice that now understands how a traditional owner can also be a cabinet minister."

'Racist and defamatory' 

In a further statement after the hearing, Ms Enoch accused the LNP of pursuing a "racist and defamatory line of questioning".

"Today, I gave members of the Queensland LNP an opportunity to support the modest offer from First Nations peoples to walk with them in support of a Voice to Parliament," her statement said.

"Each of these committee members, together with the shadow minister for Aboriginal and Torres Strait Islander Partnerships, play a role in First Nations legislation in Queensland, so I asked them to use their own voices to tell Queenslanders where they stand.

The LNP has been contacted for comment.